abstract：:Saccharomyces cerevisiae strain MW11 is a temperature-sensitive mutant which exports twenty times more proteins at 37 degrees C than parental or wild-type strains do. To understand the mechanism underlying the protein overexport in the mutant the possibility of an altered cell-wall structure leading to facilitated rel...

journal_title：Folia microbiologica

authors： Alexieva KI,Venkov PV

更新日期：2000-01-01 00:00:00

abstract：:Five trisubstituted quinazolones and eight trisubstituted quinazoline-4-thiones have been tested for antibacterial effects by a microdilution method. Four derivatives exerted a significant effect on E. coli, P. aeruginosa, S. aureus and B. subtilis (IC50 < 100 mg/L). In the bacterium P. aeruginosa six quinazolines sho...

journal_title：Folia microbiologica

authors： Jantová S,Greif G,Spirková K,Stankovský S,Oravcová M

更新日期：2000-01-01 00:00:00

abstract：:A total of 368 rotavirus RNA-positive (PAGE) stool samples collected continually during 1992-95 from infants and young children under five years of age hospitalized with acute gastroenteritis were serotyped using an enzyme immunoassay with VP7-specific monoclonal antibodies (ELISA with MAbs) for serotypes G1-G4. The s...

journal_title：Folia microbiologica

authors： Tietzová J,Petrovicová A

更新日期：2000-01-01 00:00:00

abstract：:Ovine and caprine scrapie occupies a unique place among animal transmissive spongiform encephalopathies (TSE). It is an object of intensive biomedicinal, ecological and economical studies. Its causative agents are demonstrably associated with the development of TSE in farmed minks, goats and moufflons. Ovine strains o...

journal_title：Folia microbiologica

authors： Novák M,Vrtiak OJ,Mikula I,Tkáciková L

更新日期：2000-01-01 00:00:00

abstract：:A proteus strain inhibited mycelial growth of Fusarium oxysporum in vitro. Seed bacterization showed significant plant growth promotion and Fusarium-wilt suppression activity of Phaseolus mungo in a gnotobiotic system. The culture filtrate of this strain exhibited three prominent bands in UV-VIS spectra between 300 an...

journal_title：Folia microbiologica

authors： Barthakur M

更新日期：2000-01-01 00:00:00

abstract：:Effects of increasing doses of UV-B radiation on photosynthetic efficiency (PE) and motility in Chlamydomonas reinhardtii were investigated. The exposure time ranged from 10 to 120 min at 0.73 W/m2 UV-B radiation. A decrease in compensation points (CP) was followed by an increase in PE on extending the UV-B treatment ...

journal_title：Folia microbiologica

authors： Danilov RA,Ekelund NG

更新日期：2000-01-01 00:00:00

abstract：:A new strain of Bacillus sp. was isolated from a hot water spring in India. This strain generated a high activity of extracellular beta-galactosidase at 37 degrees C in shake flasks. The beta-galactosidase activity was found to increase continuously but the production rate was slower than with some other organisms rep...

journal_title：Folia microbiologica

authors： Sani RK,Chakraborti S,Sobti RC,Patnaik PR,Banerjee UC

更新日期：1999-01-01 00:00:00

abstract：:Isolation and structure elucidation was carried out of flavonoid constituents found in fractionated extracts of the seeds and green leaves of Daucus carota L. var. boissieri (Apiaceae). The flavonoids are mainly apigenin, luteolin, their glycosidic precursors and 2,4,5-trimethoxybenzaldehyde. Fatty acids, hydrocarbons...

journal_title：Folia microbiologica

authors： Shalaby NM,Maghraby AS,el-Hagrassy AM

更新日期：1999-01-01 00:00:00

abstract：:Treatment of human group C and G streptococci with cyanogen bromide results in solubilization of surface protein G molecules. Strain-to-strain variation in the quantity, molar mass and functional activity of protein G extracted from representative group C and G isolates led to the identification of three structurally ...

journal_title：Folia microbiologica

authors： Gupalova T,Voltchek N,Totolyan A

更新日期：1999-01-01 00:00:00

abstract：:Almost all dried vegetative trichomes ofAnabaena iyengarii, Westiellopsis prolifica andNostochopsis lobatus died within 1 h, while those ofOscillatoria acuminata retained viability to some extent for 1 d under similar storage conditions. The viability of dried vegetative trichomes ofO. acuminata decreased about equall...

journal_title：Folia microbiologica

authors： Agrawal SC,Singh V

更新日期：1999-01-01 00:00:00

abstract：:Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and deletions in a small region of the sequence. In the first step, a uniq...

journal_title：Folia microbiologica

authors： Adamec J,Kalousek F

更新日期：1999-01-01 00:00:00

abstract：:The ribosomal stalk is involved directly in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes, the acidic...

journal_title：Folia microbiologica

authors： Rodriguez-Gabriel MA,Bou G,Briones E,Zambrano R,Remacha M,Ballesta JP

更新日期：1999-01-01 00:00:00

abstract：:Glutamate was converted to glutamine by Corynebacterium glutamicum permeabilized by ethanol (10%). ATP was supplied to the reaction by dried Saccharomyces cerevisiae or regenerated by acetyl kinase. High glutamine synthetase activity in C. glutamicum was induced by cultivation of the microorganism in media containing ...

journal_title：Folia microbiologica

authors： Behenský P,Stenzl V,Adámek F,Bĕhal V

更新日期：1999-01-01 00:00:00

abstract：:Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 ha...

journal_title：Folia microbiologica

authors： Sobotková L,Grafková J,Stĕpánek V,Vacík T,Maresová H,Kyslík P

更新日期：1999-01-01 00:00:00

abstract：:This study was undertaken to develop a new Gram-staining machine controlled by a micro-controller and to investigate the quality of slides that were stained in the machine. The machine was designed and produced by the authors. It uses standard 220 V AC. Staining, washing, and drying periods are controlled by a timer b...

journal_title：Folia microbiologica

authors： Felek S,Arslan A

更新日期：1999-01-01 00:00:00

abstract：:Bacteria accumulate high amounts of potassium in the cytoplasm. For studying transport of K+ (with 86Rb as a marker) in bacteria (Staphylococcus aureus 17810S), the cells were depleted of the internal K+ pool by a DNP treatment. Kinetics and energetics of 86Rb transport was assayed with glucose as an exogenous energy ...

journal_title：Folia microbiologica

authors： Tynecka Z,Malm A,Kosikowska U,Kot A

更新日期：1998-01-01 00:00:00

abstract：:The pathogenesis of bacillary dysentery can be studied at different levels of integration of the cellular components that constitute the colonic mucosal barrier. We considered the interaction of Shigella flexneri in three experimental systems that provide complementary information and a scheme of events occurring in h...

journal_title：Folia microbiologica

authors： Sansonetti PJ

更新日期：1998-01-01 00:00:00

abstract：:The resistance to killing by free radicals of two mutants ofPhaffia rhodozyma was determined. Mutant 5-7 did not produce astaxanthin but produced beta-carotene, while mutant 3-4 did not produce any carotenoid pigments. The resistance of mutant 5-7 was the same as that of the wild type but mutant 3-4 was rapidly killed...

journal_title：Folia microbiologica

authors： Santopietro LM,Spencer JF,Spencer DM,Siñeriz F

更新日期：1998-01-01 00:00:00

abstract：:Effect of oxygen and metabolic substrates (glucose, ethanol) on the catalase activity of anaerobically grown Schizosaccharomyces pombe cells was assessed and compared with that of Saccharomyces cerevisiae in order to determine the catalase activity regulation in S. pombe. In contrast to S. cerevisiae, the total catala...

journal_title：Folia microbiologica

authors： Sigler K,Gille G

更新日期：1998-01-01 00:00:00

abstract：:A high infective dose of Taxocara canis eggs (2,500 eggs per mouse) induced a partial immunosuppression in mice, manifested by inhibition of the proliferative response of splenic T and B cells to polyclonal activators. A glucan immunomodulator given to infected animals at the beginning of the experiment showed a marke...

journal_title：Folia microbiologica

authors： Borosková Z,Reiterová K,Dubinský P,Tomasovicová O,Machnicka B

更新日期：1998-01-01 00:00:00

abstract：:We have investigated the expression of cyclins, cyclin dependent kinases (CDK), and CDK inhibitors (CKI) at the mRNA level in a panel of small-cell lung cancer (SCLC) cell lines in vitro and in vivo as xenografts in nude mice. The results showed that the cell lines expressed varying amounts of most cyclin and CDK's bu...

journal_title：Folia microbiologica

authors： Krarup M,Poulsen HS,Spang-Thomsen M

更新日期：1998-01-01 00:00:00

abstract：:The aim of the study was to compare the phenotype of lymphocyte subpopulations of the GALT (gut-associated lymphatic tissue) in germfree (GF) and conventionally (CV) reared rats, i.e. to analyze the effect of microbial colonization on the development of intestinal lymphocyte subsets. Surface marker characteristics wer...

journal_title：Folia microbiologica

authors： Stĕpánková R,Sinkora J,Hudcovic T,Kozáková H,Tlaskalová-Hogenová H

更新日期：1998-01-01 00:00:00

abstract：:Escherichia coli strain HB101 harboring an expression plasmid bearing calf prochymosin gene under the control of the tac promoter was grown in the presence of IPTG with or without novobiocin at 28 and 40 degrees C, respectively. The differential rates of synthesis of prochymosin inclusions, and, for comparison, of bet...

journal_title：Folia microbiologica

authors： Kaprálek F,Tichý PJ,Fábry M,Sedlácek J

更新日期：1998-01-01 00:00:00

abstract：:Strains ofRhodotorula sp.,Candida spp. andLangermania sp. cultivated on polyunsaturated oil preferentially incorporated more unsaturated fatty acids. These fatty acids were used mainly for growth needs whereas the saturated ones accumulated in the microbial cell. The cellular oil and the remaining oil in the culture h...

journal_title：Folia microbiologica

authors： Aggelis G,Papadiotis G,Komaitis M

更新日期：1997-04-01 00:00:00

abstract：:As a part of a program to develop starter cultures aiding in the spoilage control and sanitation of African fermented foods, a cereal-based food ('ogi' and its solid form 'agidi' or 'eko') was prepared using a bacteriocin-producing Lactobacillus strain as the starter culture. The survival of an enterotoxigenic Escheri...

journal_title：Folia microbiologica

authors： Olasupo NA,Olukoya DK,Odunfa SA

更新日期：1997-01-01 00:00:00

abstract：:The postantibiotic effect (PAE) and the postantibiotic sub-MIC effect (PASME) of ciprofloxacin, pefloxacin and amikacin were studied for Salmonella typhimurium and S. enteritidis strains. PAE was induced by 2 x and 4 x MIC of antibiotics studied for 0.5 h. After PAE and PASME their effect on prophage induction of a ly...

journal_title：Folia microbiologica

authors： Majtán V,Majtánová L

更新日期：1997-01-01 00:00:00

abstract：:Pullulanase of Klebsiella oxytoca is one of a wide variety of extracellular proteins that are secreted by Gram-negative bacteria by the complex main terminal branch (MTB) of the general secretory pathway. The roles of some of the 14 components of the MTB are now becoming clear. In this review it is proposed that most ...

journal_title：Folia microbiologica

authors： Pugsley AP,Francetic O,Hardie K,Possot OM,Sauvonnet N,Seydel A

更新日期：1997-01-01 00:00:00

abstract：:The ability of Phanerochaete chrysosporium, Trametes versicolor, Coriolopsis polyzona, and Pleurotus ostreatus growing in a mitogen-limited mineral medium (NMM) to degrade PCBs in a commercial, Delor 106 mixture at a concentration of 0.9 ppm was compared. The respective amount of PCBs removed from the fungal cultures ...

journal_title：Folia microbiologica

authors： Novotný C,Vyas BR,Erbanová P,Kubátová A,Sasek V

更新日期：1997-01-01 00:00:00

abstract：:The potentiality of twenty-five fungal species belonging to 14 genera isolated from Egyptian soils to produce mevinolin, a hypocholesterolemic agent, when grown on selected substrates was tested. For the first screening samples of culture filtrates were tested by TLC and the positive results were further estimated by ...

journal_title：Folia microbiologica

authors： Shindia AA

更新日期：1997-01-01 00:00:00

abstract：:Virus-like particles (VLPs) were isolated from the yeast Wickerhamia fluorescens strain CCY61-1-1. The VLPs are approximately 42 nm in diameter and contain only one species of dsRNA molecule. The apparent length of the dsRNA determined by native agarose gel electrophoresis was 4.6 kbp. Analysis of protein content of t...

journal_title：Folia microbiologica

authors： Pospísek M,Palková Z,Korb J,Vanĕk D

更新日期：1996-01-01 00:00:00

abstract：:A decrease in the content of ochratoxin A (OA) was observed in milk samples fermented by yoghurt bacteria and bifidobacteria. OA was added to the milk before fermentation at a rate of 0.05-1.5 mg/L. No residues of OA were found in samples containing 0.05 and 0.1 mg/L of OA, fermented by S. Salivarius subsp. thermophil...

journal_title：Folia microbiologica

authors： Skrinjar M,Rasić JL,Stojicić V

更新日期：1996-01-01 00:00:00

abstract：:The effect of immersion time and cell concentration in the attachment of several lactic acid bacteria with antibacterial activity to beef-muscle surface was studied. The number of firmly attached bacteria increased with immersion time in the case of Pediococcus acidilacti, Lactobacillus sake, Lactococcus cremoris (two...

journal_title：Folia microbiologica

authors： Benito Y,Pin C,Fernández MF,Marín ML,Selgas MD,García ML,Casas C

更新日期：1996-01-01 00:00:00

abstract：:The effect of dental plaque bacteria on LFA-1 beta expression on peripheral blood leukocytes was studied in 20 patients with early-onset periodontitis and in 10 healthy controls. Stimulation of PMN with selected dental plaque bacteria which play a role in the pathogeny of periodontitis significantly increased the expr...

journal_title：Folia microbiologica

authors： Procházková J,Bártová J,Bílková A,Kruzík P,Krejsa O,Dusková J,Mrklas L

更新日期：1996-01-01 00:00:00

abstract：:Aminoglycosides at 2x or 4x minimum inhibitory concentration induced postantibiotic effects against Pseudomonas aeruginosa lasting 3.5-4.9 h (gentamicin) and 0.5-3.7 h (selemycin). Postantibiotic effects of subinhibitory concentrations of the aminoglycosides tested were substantially longer. Some combinations of supra...

journal_title：Folia microbiologica

authors： Hostacká A

更新日期：1996-01-01 00:00:00

abstract：:Propionate at concentrations up to 0.05% decreased at 25 degrees C the growth and sporulation of Penicillium aurantiogriseum. The standard size of conidiogenous structures (metulae, phialids) and conidia was diminished. The effect was more pronounced at a higher temperature (30 degrees C). Inhibition of ochratoxin pro...

journal_title：Folia microbiologica

authors： Skrinjar M,Danev M,Dimić G

更新日期：1995-01-01 00:00:00

abstract：:The effect of subinhibitory concentrations (subMICs) of new organic ammonium salts of four homologous series of alkylammonium bromides (32 compounds) was determined with respect to the induction of lysogenic strain prophage, influence of permeability reactions in a rabbit skin test and cytotoxic changes of monolayers ...

journal_title：Folia microbiologica

authors： Majtánová L,Hostacká A,Majtán V

更新日期：1995-01-01 00:00:00

abstract：:Common variable immunodeficiency (CVID) comprises a heterogeneous group of patients with as yet undefined genetic defects. Patients with CVID have in common a decrease in the levels of one or more serum immunoglobulin isotypes and a severe defect in the production of specific antibodies. Typically, the patients suffer...

journal_title：Folia microbiologica

authors： Eibl MM,Wolf HM

更新日期：1995-01-01 00:00:00

abstract：:A 6.5 kb DNA fragment containing the gene (thrC) encoding threonine synthase, the last enzyme of the threonine biosynthetic pathway, has been cloned from the DNA of Bacillus sp. ULM1 by complementation of Escherichia coli and Brevibacterium lactofermentum thrC auxotrophs. Complementation studies showed that the thrB g...

journal_title：Folia microbiologica

authors： Malumbres M,Mateos LM,Guerrero C,Martín JF

更新日期：1995-01-01 00:00:00

abstract：:Physiological and biochemical properties were tested in 45 isolates of Aspergillus egyptiacus (16 isolates), Emericella nidulans (16) and Aspergillus versicolor (13). The three fungal species exhibited common and similar features. The big similarity between A. egyptiacus and E. nidulans was greater than between A. egy...

journal_title：Folia microbiologica

authors： Zohri AA,Ismail MA

更新日期：1994-01-01 00:00:00

abstract：:Fourteen of 200 Lactobacillus isolates from African fermented foods, viz. 'wara', 'kenkey', 'ugba', 'ogi', 'kunuzarki', 'fufu' and 'iru' were found to produce bacteriocins against L. plantarum and only three bacteriocinogenic isolates inhibited some of the food pathogens. Plasmid analysis of the 14 bacteriocin-produci...

journal_title：Folia microbiologica

authors： Olasupo NA,Olukoya DK,Odunfa SA

更新日期：1994-01-01 00:00:00